Skip to main content
. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Mol Cell Neurosci. 2008 Aug 15;39(3):452–464. doi: 10.1016/j.mcn.2008.07.026

Figure 3. 3H-fucose incorporation into C45 agrin and C45 agrinS1726A mutants in Lec1 cells.

Figure 3

cDNAs for FLAG-tagged C45(z0) and C45(z8) agrin were transfected into Lec1 CHO cells labeled with 3H-fucose. Secreted proteins were then purified and analyzed for 3H-fucose incorporation by autoradiography. Both C45(z0) and C45(z8) agrin incorporated 3H-fucose, though C45(z8) less so. Mutation of serine 1726 to alanine (S1726A) in either protein eliminated 3H-fucose incorporation. Data are representative of 4 experiments with similar results.