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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Mol Cell Neurosci. 2008 Aug 15;39(3):452–464. doi: 10.1016/j.mcn.2008.07.026

Figure 5. Activation of MuSK tyrosine phosphorylation by C45(z0)S1726A.

Figure 5

(A) MuSK kinase was immunoprecipitated from C2C12 myotubes after addition of 1nM FLAG-tagged C45(z8), C45(z0), or C45(z0)S1726A agrin and probed with anti-MuSK or anti-phosphotyrosine (pTyr) antibodies by Western blot. C45(z0) agrin did not increase MuSK tyrosine phosphorylation, while C45(z0)S1726A and C45(z8) agrin did. (B) The ratio of phosphotyrosine to MuSK protein is higher for C45(z8) and C45(z0)S1726A agrin relative to C45(z0) or no agrin treatment. Errors are SD for n=3.