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. 2009 Feb 13;5(2):192–200. doi: 10.7150/ijbs.5.192

Figure 2.

Figure 2

Figure 2

Effects of BMP-13 on alkaline phosphatase (ALP) expression and matrix mineralization of osteogenic differentiated BM MSCs by cytological staining. MSC differentiation was induced in osteo-inductive medium and supplemented with 100, 300, and 500 ng/ml of BMP-13 or 100 ng/ml of BMP-7 respectively for 14-21 days. Active ALP was detected by a blue cleavage product using a Fast blue RR salt dye (A). Matrix mineralization was visualised by Alizarin red S staining at days 14 (B) and 21 (C). Undifferentiated MSCs were used as the negative control. CTL = undifferentiated negative control; OM = osteo-inductive medium; BMP-7 = OM+BMP-7; BMP-13 = OM+BMP-13 for all figures. The BMP concentrations at ng/ml are listed in brackets. Original magnification x 100 for all images. ALP activity was measured in MSCs after 14 days differentiation in osteo-inductive medium with or without BMP-13 (3-800 ng/ml). ALP activity is represented as A405 mean values from quadruplicate cultures (D).