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. 2000 Feb 4;97(4):1501–1505. doi: 10.1073/pnas.040549897

Figure 3.

Figure 3

Hfq strongly binds poly(A) RNA. Gel shift experiments were performed with RNA primers with or without a poly(A) extension indicated in a (Top) or b and c (Left). (a) Increasing amounts of Hfq (55 fmol in lanes 2, 6, 10, and 14; 275 fmol in lanes 3, 7, 11, and 15 and 1100 fmol in lanes 4, 8, 12, and 16) were added to the different primers. (b and c) The complexes formed by the RNA primers and 1100 fmol of Hfq were competed by poly(A≈300–6000) or poly(C) (Sigma) at concentrations indicated in pmols of nucleotides (Top). S and F in b show positions of the slow and fast-migrating complexes, respectively.