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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2008 Dec 24;46(5):663–671. doi: 10.1016/j.freeradbiomed.2008.12.008

Figure 6.

Figure 6

Figure 6

A) Apocynin (400μM) decreases 2-HE levels in LPS-treated FPAEC incubated in normoxia: Intracellular levels of 2-HE were quantified in FPAEC by HPLC after n-Butanol extraction. 2-HE levels decreased by almost 60% when LPS treated FPAEC were pre-treated with apocynin (0.15 vs. 0.06 nmol 2-HE/mg protein). Norm + LPS; LPS treated FPAEC incubated in normoxia, Norm + LPS + Apocynin; LPS and apocynin treated FPAEC incubated in normoxia. * - P=0.015.

B) Apocynin attenuates LPS-induced FPAEC apoptosis in normoxia: Quantification of apoptosis in FPAEC treated with LPS (10μg/mL) ± apocynin (400μM) for 48 hours in normoxia. Cells detected in early and late apoptosis for each condition were pooled and averaged from four independent experiments. Norm C; Normoxia control, Norm + Apocynin; FPAEC treated with apocynin, Norm + LPS; LPS treated FPAEC incubated in normoxia, Norm + LPS + Apocynin; LPS and apocynin treated FPAEC incubated in normoxia. * - P=0.002, ** - P<0.001, § - P=0.71.