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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Cell Immunol. 2008 Aug 30;254(2):105–109. doi: 10.1016/j.cellimm.2008.07.004

Figure 1. LAMP externalization as a marker for NK-92 lytic granule exocytosis.

Figure 1

A) Unstimulated NK-92 cells were fixed and permeabilized and stained for LAMP-1 and perforin. Scale bar denotes 10 μM. B) i) NK-92 cells were stimulated with TG and PMA and LAMP externalization was measured at various time points. Histograms of anti-LAMP fluorescence are shown. ii) Geometric mean (g.m.) from the experiment in (i) plotted vs. time. C) Representative histograms of anti-LAMP staining intensity showing the effects of stimulating cells with TG, PMA or both TG+PMA on granule release. D) Representative histograms of anti-LAMP staining intensity showing the effects of varying calcium levels on lytic granule exocytosis.