Cell phenotypes and Ab production in the BAL and lungs of immunized, RSV-challenged mice with and without depletion. Total BAL (a) and lung (d) cell recoveries were counted by trypan blue exclusion. BAL cells were stained for CD8 (b), CD4 (e), B220 (c), or DX5 (f) surface markers. BAL supernatants were stored at −70°C before total IgE (g) and IgA (h) detection using biotinylated Abs according to the manufacturer's instructions (as described in Materials and Methods). Results are mean ± SEM of five mice per group of TCRγδ-depleted mice (■) or controls (open symbols). *, p < 0.05; **, p < 0.02.