Figure 3. The Absence of C3aR and C5aR Prevents T Cell Immunity In Vivo.

(A) WT or C5ar1^−/−C3ar1^−/− mice (n = 3 per group) were immunized with ovalbumin protein mixed in IFA, and spleen cells on day 10 were assayed for responses to OVA_323–339 by ELISPOT. No response occurred with control peptides or naive mice.

(B) Analogous to the experiment in (A), animals were injected s.c. with ovalbumin mixed in PBS, and responses to OVA_323–339 were assayed on day 10.

(C) Syngeneic WT and C5ar1^−/−C3ar1^−/− male spleen cells were injected i.v. into WT or C5ar1^−/− C3ar1^−/− B6 females, respectively, and 10 days later, recipient spleen cells were assayed for responses to class II-restricted Dby peptide.

(D) WT or C5ar1^−/−C3ar1^−/− mice were infected with T gondii. All C5ar1^−/−C3ar1^−/− mice died by day 12, whereas WT animals survived for >50 days. Spleen cells from C5ar1^−/−C3ar1^−/− and WT animals isolated on day 7 or 10 were stimulated with 1 μg of Toxoplasma gondii antigen and assayed for IFNγ by ELISPOT (left) or for IL-12 by ELISA (right) (n = 5 per group each time; some dots overlap). *p < 0.05 versus controls.

(E) Clinical scores in WT and C5ar1^−/−C3ar1^−/− mice in which EAE was induced by immunization s.c. with 200 μg MOG_35–55 in CFA and 200 ng of pertussis toxin. (n = 5 each group, p < 0.05).

(F) Globes from WT and C5ar1^−/−C3ar1^−/− mice 15 days after inoculation of scratched corneas with KDS strain of herpes simplex virus (n = 5 each group). All error bars are ± SD.