Figure 2. Increased proliferative potential and altered fate of Rb family TKO early hematopoietic progenitor cells.
A Proliferation analysis of sorted pre-M cells, MP, and KLS cells (n>3). B Analysis of MP and KLS populations in control and TKO mice two months after pI-pC treatment. C Total number of KLS cells, Flk2− cells, Flk2+ cells in the BM of control and TKO mice (pooled from the four limbs) (n=5). D Total number of MP, CMP, GMP and MEP in the BM (four limbs) (n=5). E Analysis of the CLP population. F Total number of CLP cells (n=5). G Proliferation analysis of CD34+/CD34− KLS cells and CMP/GMP cells (n=3). H Methylcellulose assay with 10,000 unfractioned BM cells, 100 sorted KLS cells, and 100 double-sorted MP cells.
