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. 2000 Jan 31;97(4):1512–1515. doi: 10.1073/pnas.010426997

Figure 2.

Figure 2

Effect of mutations in the pseudoknot structure on IRES-mediated translation of PSIV. (A) Nucleotide sequence of a part of the transcript from the pT7CAT-5375 (Fig. 1B) and its site-directed mutants used for the translation assay. The numbers on the sequence represent nucleotide positions on the PSIV genome. (B) ECL detection of the translation products of the RNAs shown in A. The characters S and D preceding the sequences in A and above the lanes in B indicate mutations of a single triplet to disrupt base pair interactions and double mutations of the triplet along with its corresponding triplet to restore the base pair interactions, respectively.