Figure 3.
Evidence that methionine is not the initiating amino acid in IRES-mediated translation of PSIV. (A) The RNA constructs used in the methionine-incorporation assay. The white and black arrowheads indicate wild-type and mutated codons, respectively. Each of the RNAs was translated in RRL in the presence of biotin-lysyl-tRNA and unlabeled or [35S]methionine. (B) ECL detection of the translation products labeled with biotinylated lysine. (C) Autoradiography of the translation products labeled with [35S]methionine.