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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Microbiology (Reading). 2008 Nov;154(Pt 11):3579–3589. doi: 10.1099/mic.0.2008/021063-0

Figure 3. Protease sensitivity of WT PrfA and PrfA* mutants.

Figure 3

A. Wild type (W) and PrfA G145S (G) recombinant proteins were digested for 10, 30, 60, 90, and 120 minutes with trypsin, subjected to SDS-PAGE and visualized by Coomassie stain. Minus symbol denotes protein without trypsin, plus symbol denotes trypsin digestion of denatured protein. Lane M contains molecular weight markers. B. Wild type and PrfA* proteins were treated with trypsin for 10, 30, 60, and 120 minutes. W, wild type; L, L140F; Y1, Y63C, Y2, Y154C; E, E77K. Minus symbol indicates samples without trypsin, plus symbol indicates trypsin treated denatured protein. Numbers on left represent molecular weight in kD. Gel is representative of three similar experiments.