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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Microbiology (Reading). 2008 Nov;154(Pt 11):3579–3589. doi: 10.1099/mic.0.2008/021063-0

Figure 4. Dimer formation of PrfA and PrfA* proteins.

Figure 4

A. Purified wild type and PrfA* protein (500 ng) was incubated with 10uM EGS (E) or BS3 (B) or without crosslinking agent (-) for one hour at room temperature and subjected to SDS-PAGE and Western analysis with polyclonal antibody directed against PrfA (Greene & Freitag, 2003). Arrows indicate molecular weight in kD. B. Ratio of dimer to monomer as determined from three independent experiments. Ratios were calculated by measuring the intensity of the peptide bands and dividing the monomer values by the dimer values, and include standard deviations.