. Author manuscript; available in PMC: 2009 Nov 1.

Published in final edited form as: Microbiology (Reading). 2008 Nov;154(Pt 11):3579–3589. doi: 10.1099/mic.0.2008/021063-0

Table 2.

Oligonucleotides used in this study

Primer Name	Sequence	Characteristics
5’hly	TCCTATCTTAAAGTGACTTTTATGTT	5’ hly promoter
3’hly	GCTTCTAAAGATGAAACGCAATATTA	3’ hly promoter
5’actA	GATGCTTCTAAAAAAGTTGCTGAAGC	5’ actA promoter
3’actA	TATTCATGAATTATTTTAAGAATATCA	3’ actA promoter
+374 prfA	GCGCTGCAGGAAACTTGTTTTTGTAGGGTTTGG	3’ truncated prfA ORF
5’ prfA orf	CACCATGAACGCTCAAGCAGAA	5’ prfA ORF for protein expression
3’ prfA orf	TCCTCATTGAGGAATACTGTT	3’ prfA ORF for protein expression
prfA-trunc- nt3-F^b	GCGCTGCAGGAACGCTCAAGCAGAAG AATTC	5’ truncated prfA
prfA-trunc- nt841-R^b	GCGCTGCAGGGAACAACTATCTGTTGC AGCTC	3’ truncated prfA ORF
prfA-L140F- QkCh-F^b	GATTTTTCGATTAACGGGAAGTTTGGC TCTATTTGCGGTCAAC	5’ L140F Quikchange
prfA-L140F- QkCh-R^b	GTTGACCGCAAATAGAGCCAAACTTCC CGTTAATCGAAAAATC	3’ L140F Quikchange
prfA-G145S- QkCh-F^b	GGAAGCTTGGCTCTATTTGCAGTCAAC TTTTAATCCTGACC	5’ G145S Quikchange
prfA-G145S- QkCh-R^b	GGTCAGGATTAAAAGTTGACTGCAAAT AGAGCCAAGCTTCC	3’ G145S Quikchange