Figure 4.

Time and dose-dependent induction of StAR mRNA and protein by PGE₂ in endometriotic stromal cells. PGE₂ stimulated StAR mRNA (A) and protein levels (B) in a time- and dose-dependent manner in endometriotic stromal cells. StAR protein in the last lane in the upper panel B was detected in endometriotic stromal cells treated for 48 h with dibutyryl cAMP. mRNA or protein levels of the house-keeping genes, glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and actin, were used as loading controls.