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. 2008 Oct 9;150(2):1025–1032. doi: 10.1210/en.2008-0188

Figure 1.

Figure 1

Oatp1c1 expression in transfected HEK293 cells. A, Full-length Oatp1c1 is expressed in transfected HEK293 cells. Lysate from HEK293 cells transfected with Oatp1c1-pEF-DEST 51 (lane 1) and empty vector (lane 2) was transferred to a nitrocellulose membrane and probed with anti-V5 antibodies. B and C, Oatp1c1 is expressed at the plasma membrane (arrow) and perinuclear regions (arrowhead) in transfected HEK293 cells. Methanol-fixed HEK293 cells expressing Oatp1c1 were probed with anti-V5 antibodies to reveal V5 tagged Oatp1c1 localization. Green stain corresponds to Oatp1c1 localization.