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Schedule for investigating BALB/c and C57BL/6 mice maintained on vitamin D-deficient or normal diet and immunized three times with A-sub-Ad or Con-Ad. Also shown are the time intervals at which blood was drawn and mice were euthanized. Splenocytes were analyzed for lymphocyte subsets by flow cytometry (FACS) and responses to A-subunit protein and mitogens. Sera were characterized for vitamin D and its active metabolite, T4, and TSHR antibodies.
