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. 2008 Oct 16;150(2):1051–1060. doi: 10.1210/en.2008-1191

Figure 4.

Figure 4

TSHR antibodies, measured by TBI and ELISA induced by A-sub-Ad immunization in BALB/c and C57BL/6 mice maintained on vitamin D-deficient (D−) or -sufficient (D+) diet. Sera were tested 1 wk after two immunizations (2x) and at euthanasia after three immunizations (3x). Data are shown as the mean + sem for A-sub-Ad-injected BALB/c (n = 13 per group) and C57BL/6 (n = 17 per group) mice; Con-Ad-immunized mice, n = 4–5 mice per group. A, TBI values are shown as percent inhibition of TSH binding; B, IgG class antibody binding to TSHR A-subunit in ELISA; data are given as the OD units at 490 nm (sera diluted 1:100). Values significantly different are indicated as follows: BALB/c D− 2x vs. 3x: *, P = 0.008, t test; BALB/c D+ 2x vs. 3x: #, P = 0.010, rank sum test.