Figure 2.
Effects of other adult cell types and a β-cell line on β-cell differentiation from anlage cells. A, Histograms of the percentage of proinsulin-expressing mouse pancreatic anlage cells cultured with the indicated tissue fixed with paraformaldehyde. Data are representative of two separate experiments. The numbers in the top right hand corner refer to the percentage of cells that stain with the antiproinsulin antibody (black line) above staining with the isotype control antibody (gray line). B, The histograms show the percentages of proinsulin-expressing cells in cultures with fixed βTC3 cells (0.5 × 105 and 2 × 105 cells/well). Staining with the isotype control antibody is shown in gray. The relationship between the number of βTC3 cells added to cultures of pancreatic anlage cells and the proportion of proinsulin+ anlage cells is shown in the graph. The data are from a single experiment representative of three experiments.