Figure 5.

Effects of human adult islets on differentiation of fetal pancreatic cells. A, Real-time RT-PCR analysis of gene expression during culture of human anlage with and without fixed human islets. Bars show relative fold changes of mRNA levels of indicated gene expression in cells cocultured with fixed islets over those cultured without islets. Data are from a single experiment representative of two independent experiments. B, Flow cytometry analysis of proinsulin and Pdx-1 expression in anlage cells. Electronic gates were placed on anlage cells. The numbers refer to the percentage of proinsulin/Pdx-1+ cells. The data, shown as dot plots, are from a single experiment representative of three independent experiments. The isotype controls are on the left. **, P < 0.01; ***, P < 0.001. PE, Phycoerythrin.