Table 1.

FACS analysis of cell proliferation and differentiation in mouse pancreatic anlage cell culture

	Pancreatic anlage	Pancreatic anlage + fresh islets
Proinsulin+/BrdU− cells (%)	6.8 ± 1.1	14.2 ± 2.6a
Proinsulin+/BrdU+ cells (%)	3.5 ± 1.3	5.5 ± 1.1a
Total proinsulin+ cells (%)	9.7 ± 2.7	19.7 ± 5.0b

	Pancreatic anlage cells	Pancreatic anlage cells+ fixed islets
Proinsulin+/BrdU− cells (%)	7.3 ± 1.6	41.0 ± 8.4b
Proinsulin+/BrdU+ cells (%)	10.6 ± 2.0	21.0 ± 3.7a
Total proinsulin+ cells (%)	15.9 ± 1.8	58.5 ± 8.7b

Anlage cells were cultured with or without fresh or fixed adult islets. After 7 d of culture, dissociated pancreatic H2b: enhanced GFP anlage cells were intracellularly stained with fluorescently labeled mAbs to proinsulin and BrdU and were analyzed by FACS. Representative staining is shown in Fig. 1. The data shown represent the proportion of total anlage cells that stained (by flow cytometry) for proinsulin alone, proinsulin and BrdU, and the total proportion of cells that stain for proinsulin (the sum of the two previous groups). Data represent mean ± sem, (n = 3 and 6 experiments, respectively). 

^aP < 0.05. 

^bP < 0.01.