Figure 1.

Mean ± sem plasma cortisol concentrations in control ewes (open circles) and ewes subjected to the layered stress paradigm (black circles) in experiment 1 (A, left panel), and in control ewes (open circles), ewes subjected to the layered stress paradigm (black circles), and those pretreated with the GR antagonist, RU486, before stress (gray circles) in experiment 2 (B, right panel). The se about the mean plasma cortisol responses to the layered stress achieved in previous work (10) is illustrated by the shaded area in A. The layered stress paradigm is depicted at the top of the panels, and consisted of isolation, blindfold, and predator cues (dog).