Table 1.

Primer sets used in the detection of mRNA from single cells

Gene	Primer	Position	Sequence	Product size (bp)	Accession no.
Glyceraldehyde-3-phosphate dehydrogenase	GAPDH	F (outside)	gatggtgaaggtcggtgtg	469	NM_017008
		R	gggctaagcagttggtggt
		F (nested)	taccaggctgccttctct	360
		R	ctcgtggttcacacccatc
γ-Amino butyric acid	GAD67	F (outside)	cacaaactcagcggcataga	550	NM_017007
		R	gagatgaccatccggaagaa
		F (nested)	cacaaactcagcggcataga	149
		R	ctggaagaggtagcctgcac
Tyrosine hydroxylase	TH	F (outside)	cacctggagtattttgtgcg	1138	NM_012740
		R	cctgtgggtggtaccctatg
		F (nested)	tcgacccagtatatccgcca	376
		F.	tcggacacaaagtacacagg
Vasopressin	VP	F (outside)	cctcacctctgcctgctactt	237	NM_016992
		R	agccagctgtaccagcctaa
		F (nested)	acctctgcctgctacttcca	216
		R	agccagctgtaccagcctaa
Oxytocin	OT	F (outside)	ctgccccagtctcgcttg	281	NM_012996
		R	cctccgcttccgcaaggcttctggc
		F (nested)	ctgccccagtctcgcttg	244
		F	gcgagggcaggtagttctcc
Corticotropin-releasing hormone	CRH	F (outside)	ggggaaaggcaaagaaaagg	184	X03036
		R	gacagagccaccagcagcat
		F (nested)	ggagaagagaaaggagaagaggaa	140
		R	ggacaccagcagccgcag
Thyrotropin-releasing hormone	TRH	F (outside)	agaggggagacttgggagaa	44?	M23632
		R	ctttgcttcaccagggtctc
		F (nested)	attcatgggcagatgaggag	245
		R	ggcgtttctcaggcattaag
Vesicular glutamate transporter-2	VGLUT2	F (outside)	aggttggctaccacctcct	583	AF271235
		R	tgagagtagccaacaaccagaagca
		F (nested)	cccgcaaagcatccaacca	164
		R	cctgcagaagtttgcaacaa
Adiponectin receptor 1	AdipoRl	F (outside)	gtcccctggctctattctcct	509	BC061839
		R	agcacttggctgtgatgt
		F (nested)	tcttcctcatggctgtgatgt	223
		R	ggctcagagaagggagtcatc
Adiponectin receptor 2	AdipoR2	F (outside)	ggagccattctctgcctttc	464	NM_001037979
		R	ccagatgtcacatttgcca
		F (nested)	actgtaacccacaaccttgcttc	191
		R	tcaggaacccttctgagatgac

F, Forward; R, reverse.