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. 2008 Oct 9;150(2):849–861. doi: 10.1210/en.2008-1069

Figure 4.

Figure 4

Production of 6-keto-PGF-1α is elevated in aortas of STZ-treated mice. Aortas from mice treated with CTRL or STZ for the indicated times were prepared as described in Materials and Methods. Aortas were incubated in Krebs-Henseleit solution (20 min, 37 C) and then treated without or with NA + ACh in the absence or presence of COX-2 inhibitor NS-398 (10 μm). Conditioned media were then collected for measurement of 6-keto-PGF-1α (ELISA). Levels of 6-keto-PGF-1α were significantly increased in response to NA + ACh stimulation in conditioned media from cultured aortas isolated from mice 1 wk after injection with CTRL or STZ (vs. respective basal). *, P < 0.005; **, P < 0.001. Significant elevations of 6-keto-PGF-1α concentrations in conditioned media were observed both before and after treatment with NA + ACh in aortas isolated from mice 8 wk after injection with STZ (vs. CTRL basal. #, P < 0.01. Results are mean ± sem (normalized to wet weight of aorta) for experiments repeated independently three times in duplicate.