Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Feb 9;184(3):373–381. doi: 10.1083/jcb.200808130

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 Maresca and Salmon

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — K-Tensor Drosophila S2 cells exhibit detectable changes in the distance between the inner and outer layers of the kinetochore. (A) Representative micrographs of prophase and metaphase K-Tensor cells expressing Ndc80-GFP (green) and CID-mCherry (red). Ndc80, CID, and merged images are shown for the highlighted kinetochore pairs (white arrows). Line scans for each of the kinetochore pairs are shown to the right of each pair, with the red line indicating CID-mCherry intensity and the green line reflecting Ndc80-GFP intensity. (B) A single frame from a dual-view imaged K-Tensor cell (Video 1, available at http://www.jcb.org/cgi/content/full/jcb.200808130/DC1) showing simultaneous imaging of Ndc80-GFP and CID-mCherry. The inset shows enlarged images of the Ndc80 (green) and CID (red) signals for the highlighted kinetochore pair (white boxes). The distances between the two brightest pixels for each pair are represented by ΔNdc80 and ΔCID. (C) To calculate delta (δ), which is the distance between Ndc80-GFP and CID-mCherry, ΔCID is subtracted from ΔNdc80 and divided by two. Bars: (A and B, inset) 1 µm; (B) 10 µm.