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. 2009 Feb 9;184(3):365–372. doi: 10.1083/jcb.200809055

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© 2009 Toso et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Kinetochores and poleward MT flux are required for timely centrosome separation. (A and B) Mean bipolar spindle formation time in the prometaphase pathway in siControl (n = 38)-, siMcm21R (n = 36)-, siMCAK (n = 23)-, or siMcm21R + siMCAK (n = 31)–treated cells (A) or in siControl (n = 38)-, siNuf2R (n = 40)-, siCENP-E (n = 28)–, siMCAK + siKif2A (n = 38)–, or siCLASP1 + siCLASP2 (n = 28)–treated cells (B). (C) Representative images of siControl- or siMcm21R RNA–treated cells stained with CREST antisera (to mark kinetochores) and EB1 antisera (to mark growing MT plus ends). Asterisks indicate statistically significant differences (P < 0.01 by Mann-Whitney test). Error bars represent SEM. Bar, 10 µm.