Figure 7.
Cdc28p inhibits Fus2p-GFP nuclear exit in G2/M. Localization of GAL1-expressed Fus2p-GFP was examined as described in Fig. 1 D in cdc28-as1 (MY10324). (A) Cdc28p-as1 inhibitor was added without α factor. (B) Cdc28p-as1 inhibitor and α factor were added together. The images show cytoplasmic and cortical localization in large-budded cells before cytokinesis. The bottom panel shows a cell dying during the course of the experiment. Similar results were obtained when Fus2p-GFP was expressed under its own promoter. Bars, 1 µm.