Table II.
Plasmid | Markers | Source |
pRS413 | CEN HIS3 | Sikorski and Hieter, 1989 |
pSB231 | CEN URA3 fus1-LacZ | Trueheart et al., 1987 |
pJAU01 | URA3 cdc28-as 1 | Bishop et al., 2000 |
pSY1 | 2µ LEU2 STE12 | Dolan et al., 1989 |
pPP2330 | URA3 STE5-8A | Strickfaden et al., 2007 |
YCpIF5 | CEN URA3 PGAL1 | Foreman and Davis, 1994 |
pPROTet.E133 | Ptet-6xHN Cmr | Clontech Laboratories, Inc. |
pMR4937 | CEN URA3 FLAG-FUS3 | Matheos et al., 2004 |
pMR4938 | CEN URA3 FLAG-fus3Q93G | Matheos et al., 2004 |
pMR5048 | CEN HIS3 FLAG-FUS3 | D. Matheosc |
pMR5469a | CEN URA3 PGAL1-FUS2∷GFP104 | Paterson et al., 2008 |
pMR5482 | CEN URA3 FUS2∷GFP104 | Paterson et al., 2008 |
pMR5598 | pFa6a-mCherry-kanMX6 | S. Clarkc |
pMR5630 | Ptet-6xHN-fus21–328 Cmr | This study |
pMR5642 | CEN LEU2 FUS2∷GFP104 | This study |
pMR5774b | CEN URA3 PGAL1-fus21–104-GFP | This study |
Point mutations in FUS2∷GFP used in Fig. 5 D were created for this study and are derived from pMR5469.
Point mutations in fus21–104-GFP used in Fig. 5 (B and C) were created for this study and are derived from pMR5774.
Princeton University, Princeton, NJ.