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. 2009 Feb 16;206(2):343–357. doi: 10.1084/jem.20080950

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© 2009 Maynard et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — CpG-BMDC–mediated induction of IL-10 in CD4 T cells is Myd88 and ICOSL independent but IL-6 and IL-21 dependent. (A) Naive T cells were activated on CpG-BMDC from WT, Myd88^−/−, or Icosl^−/− mice in the presence of TGF-β and analyzed on day 3 for expression of Thy1.1 and GFP. (B) CD4 T cells were cultured with WT CpG-BMDC plus TGF-β in the presence of the additives indicated and analyzed on day 3 for expression of Thy1.1 and GFP. (C) D10 BMDC (left) were left unstimulated or stimulated for 24 h with the indicated TLR/NOD agonists. MACS purified CD4⁺Thy1.1⁺ cells (right), induced in the presence of CpG-BMDC plus TGF-β, were cultured for 24 h with Il10^−/− splenic DC in the absence or presence of OVA peptide. IL-6 was quantified by cytometric bead array and IL-21 by ELISA. Mean cytokine concentrations ±SEM are shown (ND, not detected; *, P < 0.05; ***, P < 0.001). All data are representative of at least three experiments.