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. 2009 Feb 16;206(2):275–285. doi: 10.1084/jem.20080996

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© 2009 Haines et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — PTK7⁺ CD4⁺ RTEs exhibit a selective impairment of activation-induced function after engagement of the αβ-TCR/CD3 complex and CD28 compared with PTK7⁻ naive CD4⁺ T cells. (A) Activation-dependent surface expression by PTK7⁺ CD4⁺ RTEs (dashed line) and PTK7⁻ naive CD4⁺ T cells (solid line) after stimulation with α-CD3/α-CD28 mAb beads for 12 (CD154) or 24 (CD25 and CD69) h (representative results from one of three donors). (B) Intracellular TNF-α and IL-2 expression after α-CD3/α-CD28 bead stimulation for 12 h (representative results from one of three donors). (C) IL-2 content (mean ± SD of duplicate wells, with the results representative of one of four donors) in cell culture supernatants. (D) [³H]thymidine incorporation (mean ± SD) after culture with the indicated ratio of α-CD3/α-CD28 beads/cell for 96 h in the presence and absence of rhIL-2 (representative results from one of three donors). (E) IFN-γ content (mean ± SD of n = 3 donors) in cell culture supernatants after Th1 differentiation for 72 h. *, P < 0.05 by the two-tailed unpaired Student's t test.