Figure 3. Phenotypic effects of compound 1 on type III secretion needle complex assembly.
a, Type III secretion needle complexes (NC) were purified from S. typhimurium grown in the absence (−) or presence (+) of 380 µM of compound 1. Proteins were separated by SDS-PAGE and Western Blotted with antibodies to InvG, PrgH and PrgK. b, Total membrane fractions were isolated from S. typhimurium grown in the absence (−) or presence (+) of 380 µM of compound 1. Proteins were separated by SDS-PAGE and Western Blotted with antibodies to InvG and PrgH.