Table II.
Summary of metabolic activities of SEAP-producing CHO cells under different culture conditions
| Culture conditionsa | qGlC (μmol/106 cells/h) | qLac (μmol/106 cells/h) | YLac/Glc (mol/mol) | qGln (nmol/106 cells/h) | qAmm (nmol/106 cells/h) | YAmm/Gln (mol/mol) | Lactate concentration at harvest (mM) | Ammonia concentration at harvest (mM) |
|---|---|---|---|---|---|---|---|---|
| Suspension | 0.21±0.02 | 0.35±0.04 | 1.67±0.03 | 31.5±4.1 | 34.1±3.7 | 1.08±0.03 | 22 | 2.0 |
| Microcarrier (Cytodex 3) | 0.59±0.06 | 1.12±0.13 | 1.90±0.03 | 59.5±5.7 | 59.9±11.2 | 1.01±0.09 | 68 | 2.0 |
| +181%b | +220% | +14% | +88% | +76% | -6% | |||
| Microcarrier (Cytopore 1) | 0.27±0.02 | 0.51±0.05 | 1.89±0.04 | 33.3±3.1 | 29.5±2.0 | 0.89±0.03 | 38 | 1.8 |
| +29% | +46% | +13% | +6% | -13% | -18% |
| Biphasic suspension culture | Beforec | Afterd | Before | After | Before | After | Before | After | Before | After | Before | After | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.28 | 0.12 | 0.51 | 0.17 | 1.8 | 1.4 | 18 | 1.2 | 17 | 1.4 | 0.9 | 1.2 | 57 | 1.8 | |
| +33% | -43% | 46% | -51% | +8% | -16% | -43% | -96% | -50% | -96% | -17% | +11% |
a
Values are mean of two independent experiments except biphasic suspension culture.
b
Percent change compared to single temperature suspension culture.
c
Before low temperature shift.
d
After low temperature shift.