Table III.
Sugars detected in positive ion mode
| Calculated m/za [M + Na]+ | Observed m/za [M+Na]+ |
Percentage of glycans with indicated sequences from each culture condition |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Suspension | Cytodex 3 | Cytopore 1 | Biphasic suspension |
Composition/sequence | Putative structureb |
Suspension | Cytodex 3 | Cytopore 1 | Biphasic suspension |
|
| 1136.4 | 1136.9 | n.d. | n.d. | n.d. | (HexNAc)1 + (Man)3(GlcNAc)2 | A1 | 7.6 | n.d. | n.d. | n.d. |
| 1257.4 | n.d. | n.d. | n.d. | 1257.7 | (Hex)2 + (Man)3(GlcNAc)2 | A0M2 | n.d. | n.d. | n.d. | 6.6 |
| 1282.5 | 1283.5 | 1283.1 | 1282.7 | 1282.8 | (HexNAc)1(Deoxyhexose)1 + (Man)3(GlcNAc)2 | A1F1 | 11.9 | 0.8 | 2.4 | 4.7 |
| 1298.4 | 1299.4 | n.d. | n.d | 1298.9 | (Hex)1(HexNAc)1 + (Man)3(GlcNAc)2 | A1G1 | 3.5 | n.d. | n.d. | 12.7 |
| 1419.5 | 1420.4 | n.d. | n.d | 1419.9 | (Hex)3 + (Man)3(GlcNAc)2 | A0M3 | 6.7 | n.d. | n.d. | 4.9 |
| 1444.5 | 1443.2 | n.d. | 1445.7 | 1444.9 | (Hex)1(HexNAc)1(Deoxyhexose)1 + (Man)3(GlcNAc)2 | A1G1F1 | 4.1 | n.d. | 5.2 | 6.1 |
| 1460.5 | 1459.1 | n.d. | n.d. | 1461.1 | (Hex)2(HexNAc)1 + (Man)3(GlcNAc)2 | A1M1G1 | 1.3 | n.d. | n.d. | 5.2 |
| 1501.5 | 1502.3 | n.d. | n.d. | 1502.0 | (Hex)1(HexNAc)2 + (Man)3(GlcNAc)2 | A2G1 | 3.5 | n.d. | n.d. | 6.5 |
| 1581.5 | 1582.0 | n.d. | n.d. | 1582.0 | (Hex)4 + (Man)3(GlcNAc)2 | A0M4 | 2.7 | n.d. | n.d. | 5.6 |
| 1647.6 | 1648.2 | 1648.0 | n.d. | 1646.1 | (Hex)1(HexNAC)2(Deoxyhexose)1 + (Man)3(GlcNAc)2 | A2G1F1 | 4.8 | 1.3 | n.d. | 5.8 |
| 1663.6 | 1664.1 | 1664.1 | 1664.8 | 1664.1 | (Hex)2(HexNAc)2 + (Man)3(GlcNAc)2 | A2G2 | 5.2 | 19.4 | 3.0 | 14.8 |
| 1809.6 | 1811.1 | 1810.0 | 1810.0 | 1810.2 | (Hex)2(HexNAc)2(Deoxyhexose)1 + (Man)3(GlcNAc)2 | A2G2F1 | 12.4 | 38.3 | 76.1 | 8.3 |
| 1825.6 | 1826.1 | 1826.9 | 1825.9 | 1826.0 | (Hex)3(HexNAc)2 + (Man)3(GlcNAc)2 | A2M1G2 | 4.3 | 1.3 | 5.1 | 6.8 |
| 1866.7 | 1866.0 | n.d. | n.d. | n.d. | (Hex)2(HexNAc)3 + (Man)3(GlcNAc)2 | A3G2 | 5.0 | n.d. | n.d. | n.d. |
| 1907.7 | 1907.5 | n.d. | n.d. | n.d. | (Hex)1(HexNAc)4 + (Man)3(GlcNAc)2 | A4G3 | 1.4 | n.d. | n.d. | n.d. |
| 2028.7 | 2029.7 | 2029.5 | n.d. | 2028.8 | (Hex)3(HexNAc)3 + (Man)3(GlcNAc)2 | A3G3 | 7.2 | 10.3 | n.d. | 5.5 |
| 2174.8 | 2176.0 | 2175.5 | 2175.8 | 2175.1 | (Hex)3(HexNAc)3(Deoxyhexose)1 + (Man)3(GlcNAc)2 | A3G3F1 | 5.3 | 20.9 | 8.2 | 4.2 |
| 2231.8 | 2232.0 | n.d. | n.d. | n.d. | (Hex)3(HexNAc)4 + (Man)3(GlcNAc)2 | A4G3 | 8.4 | n.d. | n.d. | n.d |
| 2539.9 | 2541.2 | 2540.2 | n.d. | 2540.2 | (Hex)4(HexNAc)4(Deoxyhexose)1 + (Man)3(GlcNAc)2 | A4G4F1 | 4.7 | 7.7 | n.d. | 2.3 |
a
Mass (m/z) is monoisotopic mass.
b
A, N-acetylglucosamine; M, mannose; F, fucose; G, galactose.
n.d., not detected.