. Author manuscript; available in PMC: 2009 Feb 23.

Published in final edited form as: Biotechnol Bioeng. 2008 Aug 15;100(6):1178–1192. doi: 10.1002/bit.21853

Table IV.

Sugars detected in negative ion mode

Calculated m/z^a [M + Na-H]^- or [M-H]^-	Observed m/z^a[M + Na-H]^- or [M-H]^-						Percentage of glycans with indicated sequences from each culture condition
Calculated m/z^a [M + Na-H]^- or [M-H]^-	Suspension	Cytodex 3	Cytopore 1	Biphasic suspension	Composition/sequence	Putative structure^b	Suspension	Cytodex 3	Cytopore 1	Biphasic suspension
1281.5	1281.6	n.d.	1283.0	n.d.	(HexNAc)1(Deoxyhexose)1 + (Man)3(GlcNAc)2	A1F1	4.3	n.d.	19.8	n.d.
1443.5	1445.0	n.d.	n.d	n.d.	(Hex)1(HexNAc)1(Deoxyhexose)1 + (Man)3(GlcNAc)2	A1G1F1	6.0	n.d.	n.d.	n.d.
1662.6	1664.0	1664.1	1664.1	n.d.	(Hex)2(HexNAc)2 + (Man)3(GlcNAc)2	A2G2	12.6	18.4	14.3	n.d.
1808.6	1810.0	1810.2	1810.1	1809.9	(Hex)2(HexNAc)2(Deoxyhexose)1 + (Man)3(GlcNAc)2	A2G2F1	40.8	38.2	44.4	83.0
1824.6	1826.1	1826.1	1826.1	1826.0	(Hex)3(HexNAc)2 + (Man)3(GlcNAc)2	A2M1G2	16.8	12.5	14.4	17.0
2099.7	2098.9	n.d.	n.d.	n.d.	(Hex)2(HexNAc)2(Deoxyhexose)1(NeuAc)1 + (Man)3(GlcNAc)2	A2G2F1S1	9.3	n.d.	n.d.	n.d
2447.9	2449.4	2449.3	2449.0	n.d.	(Hex)2(HexNAc)3(NeuAc)2 + (Man)3(GlcNAc)2	A3G2S2	3.0	4.5	7.1	n.d.
2173.8	n.d	2175.3.	n.d.	n.d.	(Hex)3(HexNAc)3(Deoxyhexose)1 + (Man)3(GlcNAc)2	A3G3F1	n.d.	15.8	n.d.	n.d
2464.9	2465.1	n.d.	n.d.	n.d.	(Hex)3(HexNAc)3(Deoxyhexose)1(NeuAc)1 + (Man)3(GlcNAc)2	A3G3F1S1	2.4	n.d.	n.d.	n.d.
2538.9	2539.9	2540.8	n.d.	n.d.	(Hex)4(HexNAc)4(Deoxyhexose)1 + (Man)3(GlcNAc)2	A4G4F1	4.8	8.6	n.d.	n.d.
2812.9	n.d.	2814.4	n.d.	n.d.	(Hex)3 (HexNAc)4 (NeuAc)2 + (Man)3 (GlcNAc)2	A4G3S2	n.d.	2.0	n.d.	n.d.

Mass (m/z) is monoisotopic mass.

A, N-acetylglucosamine; M, mannose; F, fucose; G, galactose.

n.d., not detected.