Figure 8.

Transcriptional activity of L2-3 and L11-12 mutant SF-1 proteins is diminished. A, Induction of three SF-1 targets (inhibin α, Cyp11A1, and StAR) was determined by RT-qPCR after wild-type (WT) and mutant SF-1 expression vectors were transfected into human JEG-3 placental cells. The relative expression of all three target genes was normalized to the control parent vector (pCI-neo, indicated). B, Expression of all SF-1 variant proteins was equivalent as judged by Western blot analysis (upper panel); the control parent vector is shown in the first lane. The actin loading control is shown in the lower panel.