Figure 5.
DNA binding of the PR is important but not absolutely necessary for progestin regulation of FKBP5. HeLa cells were transfected with either the wild type (Wt) hPRB or a DNA-binding mutant (Mut) hPRB along with either an MMTV promoter-containing plasmid (pLTRLuc) or an FKBP5 construct (pGL3(−340/+1244). Cells were harvested 24 h after R5020 treatment and assayed for luciferase activity, and results were normalized for β-galactosidase activity. Data are presented as fold induction over vehicle-treated cells. Results are presented as the mean ± se of duplicates of three independent experiments.