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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Immunol Rev. 2008 Oct;225:212–225. doi: 10.1111/j.1600-065X.2008.00676.x

Fig. 5. C1q modulates mAb enhancement of WNV infection.

Fig. 5

(A). Serial dilutions of E16 (mouse IgG2b) were mixed with PBS, 5% fresh or heat-inactivated mouse serum, incubated with WNV RVP, and added to FcγRIIa+ K562 cells. Forty-eight hours later, cells were analyzed by flow cytometry for GFP expression. The data are expressed as the fold enhancement of infection compared to no antibody. (B). Experiments were performed as in panel (A) except that fresh C1q−/− or C3−/− serum or purified C1q was mixed with the mouse E16 mAb. (C, D). Experiments were performed as in panel (B) except the epitope matched DIII-specific (C) E24 (mouse IgG2a) or (D) E34 (mouse IgG1) mAbs were used. Adapted from (126).