Fig. 8. Comparing levels of activating and repressive histone modifications on the pS2 and pS2-luc sites in the ERE region and the distal 3’ regions of pS2 and pS2-luc promoters.
ChIP analysis for acetylated histone H3 (A), acetylated histone H4 (B), H3K4me3 (C), H3K27me3 (D), H3K9me3 (E), pol II (F) and total Histone H3 (G) with IgG as a negative control was performed on cells treated for 2 hr with E2 or 0.1% EtOH vehicle. The data represent the mean +/− standard errors for four independent experiments for A–C, two independent experiments for D, three independent experiments for E and F, and five independent experiments for G.