Skip to main content
. Author manuscript; available in PMC: 2009 Sep 10.
Published in final edited form as: Exp Cell Res. 2008 Jul 15;314(15):2796–2810. doi: 10.1016/j.yexcr.2008.07.006

Fig. 9. LacZ-Zeocin gene is still highly expressed in 27-9 cells and the mRNA is polycistronic to pS2-luc.

Fig. 9

A, 27-9 cells and ER27 cells were all treated with EtOH for 24 hours after being in hormone free media for 4 days. Total RNA was isolated and quantitative RT-PCR performed on the pS2-luc, pS2 and lacZ-Zeocin genes for 27-9 cells and lacZ-Zeocin gene for ER27 cells. Results were normalized to L19 that was used as loading control. Results were then expressed as fold change over one of the pS2 expression results of 27-9 cells. Data represent the means +/− standard errors of three experiments. B, RNA was isolated from 24 hour EtOH treated ER27 and 27-9 cells and converted to cDNA. Conventional PCR was performed with primers spanning the pS2-luc gene and the lacZ-Zeocin gene. The expected product length of recombinant region in 27-9 cells was polycistronic was ~4.6kb. 27-9 genomic DNA was used as a positive control and ER27 cDNA was used as a negative control