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Knockdown of PAPA-1 expression enhances IGFBP-2-induced proliferation. Endogenous PAPA-1 levels were decreased in LNCaP cells by transfection with PAPA-1 siRNA. A, Analysis of mRNA and protein by RT-PCR and Western blot, respectively. β-Actin was simultaneously evaluated as a loading control (ctrl). B, Cell viability/proliferation was assessed by the CellTiter 96 AQueous One Cell Proliferation ELISA after 72 h in serum-containing media (n = 3).
