Figure 6.

PAPA-1 inhibition of cell proliferation is IGFBP-2 dependent. A, The expression of IGFBP-2, PAPA-1, and β-actin (loading control) in IGFBP-2 knockout and wild-type MEFs was assessed by immunoblotting (IB). B, Cell proliferation of IGFBP-2 knockout and wild-type MEFs was compared after 96 h by MTS assay. C, Expression of IGFBP-2, PAPA-1, and β-actin (loading control) was assessed in MEFs transiently transfected with FLAG-PAPA-1 or empty vector control. D, BrdU incorporation as a measure of cell proliferation was assessed in MEFs transfected as in C. Data are presented compared with control, means ± sd. *, Significance that mean is different from 1: P < 0.05.