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GnRH-stimulated endogenous LHβ transcription is inhibited by MG-132. LβT2 cells were treated with 100 nm GnRH for times indicated, without (control, top) or with 50 μm MG-132 (+MG-132, bottom). LHβ PT were then measured using intron/exon-spanning primers and quantitative real-time PCR. Data are normalized to GAPDH mRNA levels and expressed as fold change vs. 0 min minus MG-132. Error bars reflect sem for triplicate samples in three experiments. *, P < 0.05 vs. control.
