Figure 2.

Electrophoretic analyses of wild-type factor V and recombinant factor V molecules. (A) Factor V^Wt and factor V^Δ680−709 were activated with RVV-V activator. (B) Factor V^Wt and factor V^4A were activated with α-thrombin as described in and analyzed by SDS−PAGE. After being transferred to a PVDF membrane, immunoreactive fragments were detected with monoclonal antibodies αHFVa_HC17 (recognizing an epitope on the heavy chain of the cofactor between amino acid residues 307 and 506) and αHFVa_HC9 (recognizing the light chain). At the right, the positions of the heavy and light chains of factor Va are shown.