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. 2008 Jul 1;47(30):7963–7974. doi: 10.1021/bi800593k

Figure 7.

Figure 7

Gel electrophoretic analyses for cleavage of FPR-meizothrombin. FPR-meizothrombin (1.4 μM) was incubated in different mixtures with PCPS vesicles (20 μM) and factor Va as described in the legend of Figure 4. The reaction and the samples were further treated, scanned, and quantified as detailed in : (A) control, no factor Va [13.4 mol of FPR-meizo consumed s−1 (mol of factor Xa)−1], (B) factor VaRVVPLASMA [42.6 mol of FPR-meizo consumed s−1 (mol of factor Xa)−1], (C) factor VaRVVΔ680−709 [8.4 mol of FPR-meizo consumed s−1 (mol of factor Xa)−1], and (D) factor VaRVV4A [14.6 mol of FPR-meizo consumed s−1 (mol of factor Xa)−1]. Lane M contained the molecular weight markers (from top to bottom): 50000, 36000, and 22000. Lanes 1−19 contained samples from the reaction mixture before (0 min) the addition of factor Xa and 20 s, 40 s, 60 s, 80 s, 100 s, 120 s, 140 s, 160 s, 180 s, 200 s, 220 s, 240 s, 5 min, 6 min, 10 min, 20 min, 30 min, and 60 min, respectively, following the addition of factor Xa. The prothrombin-derived fragments are shown as detailed in the legend of Figure 4. The factor Va species used for the reconstitution of prothrombinase are also shown under each panel.