Table 1. Strains Used in This Work.
| strain | genotype | ref |
|---|---|---|
| SS328 | MATα ade2-101 his3Δ200 ura3-52 lys2-801 | 50 |
| YG248 | MATa ade2-101 ura3-52 his3Δ200 lys2-801 Δalg3::HIS3 | 51 |
| YG1056 | MATα ade2-101 ura3-52 his3Δ200 Δalg12::kanMX4 Δalg5::HIS3; SS328/SS330 strain background | Aebi strain collection |
| YG1057 | MATa ade2-201 ura3-52 his3Δ200 lys2-801 Δalg3::HIS3 Δalg5::HIS3; SS328/SS330 strain background | Aebi strain collection |
| YG1128 | MATa/α ade2-201/ade2-201 ura3-52/ura3-52 his3Δ200/his3Δ200 tyr1/+ lys2/+ Δrft1::HIS3/+; SS328/SS330 strain background | Aebi strain collection |
| YCF40a | MATα ade2-201 ura3-52 his3Δ200 tyr1 Δrft1::HIS3 pJH19 | this study |
| BJ2168 | MATa leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2 | 52 |
a
The genomic copy of RFT1 is disrupted in strain YCF40; YCF40 contains URA3 plasmid pJH19 that encodes Rft1 tagged at its C-terminus with Protein A. We refer to this chimeric protein as Rft1*. YCF40 cells do not grow on minimal medium containing 5-fluoroorotic acid and uracil, indicating that the plasmid-encoded Rft1* protein is functional and required to complement the lack of RFT1 in the genome.