Figure 1.

(A) Schematic for the integrated proteomic approach for the identification of c-Src kinase substrates. Human embryonic kidney (HEK) 293T cells growing in Arg ‘0’ containing medium were transiently transfected with a kinase-dead Src (K298M) and 293T cells growing in Arg ‘6’ and Arg ‘10’ were transiently transfected with constitutively active Src kinase (Y527F). Arg ‘0’ refers to ¹²C₆-arginine, Arg ‘6’ refers to ¹³C₆-arginine and Arg ‘10’ refers to ¹³C₆-¹⁵N₄-arginine, isotopic labeled forms of arginine used to differentially label 293T cells for identification of Src substrates.