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MS/MS spectra of albumin peptides labeled with FP-biotin on Tyrosine. (A) Tyr 148 in peptide HPYFYAPELLFFAK is labeled with FP-biotin. The quadruply charged parent ion has a mass of 579.7 m/z. The FP-biotin tyrosine immonium ion is at 708.5; after neutral loss of 226, its mass is 482.9. (B) Tyr 401 in peptide QNCELFEQLGEYK is covalently modified by FP-biotin. The triply charged parent ion in B has a mass of 744.2 m/z.
