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. 2008 Aug 16;21(9):1787–1794. doi: 10.1021/tx800144z

Figure 4.

Figure 4

MS/MS spectrum of albumin peptide labeled with FP-biotin on tyrosine 452. The quadruply charged parent ion has a mass of 773.3 m/z. The carbamidomethylated cysteine is indicated as CAM. Internal fragmentation at proline yielded the 458.2 ion for PC(CAM)AE and the 573.2 ion for PC(CAM)AED. The characteristic fragments of FP-biotin at 227.2, 312.2, and 329.4 are present. The FP-biotin-tyrosine immonium ion is at 708.4 amu. After loss of an amine, the FP-biotin tyrosine immonium ion has a mass of 691.4 amu.