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MS/MS spectrum of albumin peptide labeled with FP-biotin on Ser 232. This spectrum was acquired on the QSTAR elite mass spectrometer. The doubly charged parent ion is at 726.9 amu. The peak at 591.3 is FP-biotin released from serine, carrying a hydroxyl group in place of fluorine. Four y-ions (y2, y3, y4, and y5) carry FP-biotin on serine, whereas six y-ions (Δy3−Δy8) have lost FP-biotin as well as a molecule of water, thus converting serine to dehydroalanine.
