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. 2008 Aug 16;21(9):1787–1794. doi: 10.1021/tx800144z

Figure 8.

Figure 8

MALDI-TOF spectrum of pepsin-digested albumin to show labeling of Tyr 411 by FP-biotin. Pepsin digestion of albumin yields two unlabeled peptides at 1717.2 (VRYTKKVPQVSTPTL) and 1830.3 (LVRYTKKVPQVSTPTL) amu, both containing Tyr 411. Both peptides have a mass shift of 572.3 after reaction with FP-biotin, yielding the peaks at 2289.6 and 2402.7 amu. The FP-biotin is covalently bound to Tyr 411. About 50% of the Tyr 411 is labeled as calculated from cluster areas of the labeled and unlabeled peaks.